A tutorial dedicated to the use of FROGS using its command line interface on the migale server is proposed here : https://tutorials.migale.inra.fr/posts/frogs-16s/. From a public dataset (Illumina 16S V3-V4), you will be able to run the full FROGS pipeline to create a BIOM file containing the OTUs, their abundances and their taxonomic affiliations. You will then learn how to deal with multi-affiliations and pick one using affiliationexplorer and how to explore community composition, alpha and beta diversities and much more with easy16S.